Journal: Nutrients

Article Title: Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets

doi: 10.3390/nu12072115

Figure Lengend Snippet: Sow diet prebiotic supplementation impacted ileal and jejunal PP cell numbers, but not the proportion of resident and migrating APC. Datasets were analyzed using the non-parametric Mann - Whitney test, for each age. ( A ) Numeration of mononuclear immune cells (×10 6 per g tissue) in il-PP of CTRL- ( n = 4, empty black bar) and PREB ( n = 4, black hatched grey bar) piglets at PND10, PND21, PND49 and PND90. ( B ) CD16+ MHCII+ proportion (% of total immune cells measured by flow cytometry) in CTRL- and PREB il-PP at PND10, PND21, PND49 and PND90. ( C ) CD16+ MHCII+ proportion (% of total immune cells) in CTRL- ( n = 4, empty black bar) and PREB ( n = 4, black hatched grey) jej-PP at PND49 and PND90. ( D ) CD11R1+ MHCII+ proportion (% of total immune cells) in CTRL- ( n = 4, empty black bar) and PREB ( n = 4, black hatched grey bar) il-PP at PND10, PND21, PND49 and PND90. ( E ) CD11R1+ MHCII+ proportion (% of total immune cells) in CTRL- ( n = 4, empty black bar) and PREB ( n = 4, black hatched grey bar) jej-PP at PND49 and PND90.

Article Snippet: Cells were incubated with primary mouse mAbs (20 min, 4 °C) recognizing porcine cell surface antigens: MHC class II (MHCII, SLA-DQ; clone MSA3, mouse IgG2a, Washington State University Monoclonal Antibody Centre, Pullman, USA) with CD16 (clone G7, mouse IgG1) or CD11R1 (CD11b; clone MIL4, mouse IgG1), both purchased from Bio-Rad (AbD Serotec, Oxford, UK).

Techniques: MANN-WHITNEY, Flow Cytometry

Journal: Nutrients

Article Title: Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets

doi: 10.3390/nu12072115

Figure Lengend Snippet: Sow diet mycotoxin contamination (DON) and neonate mycotoxin gavage (DONgav) modified jej-LP cell numbers and the proportion of resident APC. Datasets were analyzed with the non-parametric Kruskal - Wallis test followed by Dunn’s multiple comparison tests when appropriate for each age. ( A ) Numeration of immune mononuclear cells (× 10 6 per g of tissue) isolated from jej-LP at PND10, PND21 and PND49 from CTRL- ( n = 4, empty black bar), DONgav ( n = 4, light grey bar) and DON ( n = 4, dark grey bar) piglets (# p < 0.10). ( B ) CD16+ MHCII+ proportion (% of total immune cells) in jej-LP CTRL- ( n = 4, empty black bar), DONgav ( n = 4, light grey bar), and DON ( n = 4, dark grey bar) explants (* p < 0.02). ( C ) CD11R1+ MHCII+ proportion (% of total immune cells) in jej-LP CTRL- ( n = 4, empty black bar), DONgav ( n = 4, light grey bar) and DON ( n = 4, dark grey bar).

Article Snippet: Cells were incubated with primary mouse mAbs (20 min, 4 °C) recognizing porcine cell surface antigens: MHC class II (MHCII, SLA-DQ; clone MSA3, mouse IgG2a, Washington State University Monoclonal Antibody Centre, Pullman, USA) with CD16 (clone G7, mouse IgG1) or CD11R1 (CD11b; clone MIL4, mouse IgG1), both purchased from Bio-Rad (AbD Serotec, Oxford, UK).

Techniques: Modification, Comparison, Isolation